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Image Search Results


Identification of 16 cell clusters with diverse annotations reveals a high abundance of PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: Identification of 16 cell clusters with diverse annotations reveals a high abundance of PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Control, Expressing, Immunofluorescence

PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium exhibit enhanced migration and invasion capabilities. ( A ) Flow cytometric analysis of PDPN and CD90 double-positive primary FLSs populations in OA (up) and PVNS (down) synovium. ( B ) PDPN and CD90 immunofluorescence image of OA FLSs and PVNS FLSs. Red: CD90, Green: PDPN, Blue: nucleus. ( C ) Transwell migration (lower panel) and invasion (upper panel) assays of OA FLSs and PVNS FLSs. ( D ) Statistical analysis of transwell migration (lower panel) and invasion (upper panel) assays. ( E ) Wound-healing analysis of OA FLSs (up) and PVNS FLSs (down). ****P < 0.0001.

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium exhibit enhanced migration and invasion capabilities. ( A ) Flow cytometric analysis of PDPN and CD90 double-positive primary FLSs populations in OA (up) and PVNS (down) synovium. ( B ) PDPN and CD90 immunofluorescence image of OA FLSs and PVNS FLSs. Red: CD90, Green: PDPN, Blue: nucleus. ( C ) Transwell migration (lower panel) and invasion (upper panel) assays of OA FLSs and PVNS FLSs. ( D ) Statistical analysis of transwell migration (lower panel) and invasion (upper panel) assays. ( E ) Wound-healing analysis of OA FLSs (up) and PVNS FLSs (down). ****P < 0.0001.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Migration, Immunofluorescence

PDPN(+) CD90(+) sublining fibroblasts express higher levels of pro-inflammatory cytokines and invasion related proteins. ( A ) Volcano plot of DEGs in PDPN(+) CD90(+) sublining fibroblasts between the OA and PVNS synovium. ( B ) KEGG pathway analysis of the DEGs. ( C ) UMAP plots showing the expression of inflammatory factors in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( D ) UMAP plots showing the expression of osteoclast-related proteins in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( E ) Representative images of hub protein expression detected by Western blot. ( F ) Statistical analysis of Western blot. All data are expressed as mean ± SD, n = 3. ns P > 0.05; ***P < 0.001; ****P < 0.0001.

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: PDPN(+) CD90(+) sublining fibroblasts express higher levels of pro-inflammatory cytokines and invasion related proteins. ( A ) Volcano plot of DEGs in PDPN(+) CD90(+) sublining fibroblasts between the OA and PVNS synovium. ( B ) KEGG pathway analysis of the DEGs. ( C ) UMAP plots showing the expression of inflammatory factors in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( D ) UMAP plots showing the expression of osteoclast-related proteins in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( E ) Representative images of hub protein expression detected by Western blot. ( F ) Statistical analysis of Western blot. All data are expressed as mean ± SD, n = 3. ns P > 0.05; ***P < 0.001; ****P < 0.0001.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Expressing, Western Blot